Christopher A. Smith, Gregory J. Sonneson, Robert J. Hoey, Jennifer M Hinerman, Kimberly Sheehy, Richard Walter, Andrew B. Herr, and James R. Horn (2023)
Molecular recognition requires dimerization of a VHH antibody
mAbs, in press

Tosha M. Laughlin and James R. Horn (2022)
Engineering pH-Sensitive Single-Domain Antibodies
Single-Domain Antibodies: Methods and Protocols, Methods in Molecular Biology 2446

Joy M. Blain, Dakota L. Grote, Sydney M. Watkins, Gashaw M. Goshu, Chanté Muller, James L. Gorman, Gina Ranieri, Richard L. Walter, Heike Hofstetter, James R. Horn,and Timothy J. Hagen (2021)
Structural and Biophysical Characterization of the Burkholderia pseudomallei IspF Inhibitor L-Tryptophan Hydroxamate
Bioorganic & Medicinal Chemistry Letters, 48, 128273

Yashodeep Patil, Kevin Dotseth, Theodore Shapiro, Dhakshenan Pushparajan, Stephen Binderup, James R. Horn, and Venumadhav Korampally (2021)
Modular design of paper based switches for autonomous lab-on paper micro devices
Biomedical Microdevices volume 23, Article number: 1

Robert J Hoey, Hyeyoung Eom, and James R Horn
Structure and development of single domain antibodies as modules for therapeutics and diagnostics (2019)
Experimental Biology and Medicine, 244(17):1568-1576.

Watkins, Sydney M., Ghose, Debarati, Blain, Joy M., Grote, Dakota L., Luan, Chi-Hao, Clare, Michael, Meganathan,R., Horn, James R., and Hagen, Timothy J. Antibacterial activity of 2-amino-4-hydroxypyrimidine-5-carboxylates and binding to Burkholderia pseudomallei 2-C-methyl-D-erythritol-2,4-cyclodiphosphate synthase (2019) Bioorganic & Medicinal Chemistry Letters, 29(20), 126660.

Travis R. Helgren, Elif S. Seven, Congling Chen, Thomas E. Edwards, Bart L. Staker, Jan Abendroth, Peter J. Myler, James R. Horn, Timothy J. Hagen (2018)
The identification of inhibitory compounds of Rickettsia prowazekii methionine aminopeptidase for antibacterial applications
Bioorganic & Medicinal Chemistry,28, 1376-1380

Somnath Mukherjee, Dionne H Griffin, James R Horn, Shahir S Rizk, Malgorzata Nocula-Lugowska, Magnus Malmqvist, Sangwoo S Kim, and Anthony A Kossiakoff (2018)
Engineered synthetic antibodies as probes to quantify the energetic contributions of ligand binding to conformational changes in proteins
Journal of Biological Chemistry,293, 2815-2828

Travis R. Helgren, Congling Chen, Phumvadee Wangtrakuldee, Thomas E. Edwards, Bart L. Staker, Jan Abendroth,  Banumathi Sankaran, Nicole A. Housley, Peter J. Myler, Jonathon P. Audia, James R. Horn, and Timothy J. Hagen (2017)
Rickettsia prowazekii methionine aminopeptidase as a promising target for the development of antibacterial agents
Bioorganic & Medicinal Chemistry, 25, p.813–824.

Kaitlynn R. Davenport, Christopher A. Smith, Heike Hofstetter, James R. Horn, Oliver Hofstetter (2016)
Site-directed immobilization of a genetically engineered anti-methotrexate antibody via an enzymatically introduced biotin label significantly increases the binding capacity of immunoaffinity columns
Journal of Chromatography B, 1021: 114–121

Sean W. Fanning, Richard Walter, James R. Horn (2014)
Structural Basis of an Engineered Dual-Specific Antibody: Conformational Diversity Leads to a Hypervariable Loop Metal Binding Site
Protein Engineering Design and Selection 27 (10): 391-397.

Zheng Zhang, Sriram Jakkaraju, Joy Blain, Kenneth Gogol, Lei Zhao, Robert C. Hartley, c, Courtney A. Karlsson, Bart L. Staker, Lance J. Stewart, Peter J. Myler, Michael Clare, Darren W. Begley, James R. Horn, Timothy J. Hagen (2013)
Cytidine derivatives as IspF inhibitors of Burkolderia pseudomallei
Bioorganic and Medicinal Chemistry Letters, 23(24),pp 6860–6863

Phumvadee Wangtrakuldee, Matthew S. Byrd, Cristine G. Campos, Michael W. Henderson, Zheng Zhang, Michael Clare, Ali Masoudi, Peter J. Myler, James R. Horn, Peggy A. Cotter, and Timothy J. Hagen (2013)
Discovery of Inhibitors of Burkholderia pseudomallei Methionine Aminopeptidase with Antibacterial Activity
ACS Med. Chem. Lett., 2013, 4 (8), pp 699–703

Sztuba-Solińskaa,J., Fanning, S.W., Horn, J.R., and Bujarski, J.J. (2012)
Mutations in the coat protein-binding cis-acting RNA motifs debilitate RNA recombination of Brome mosaic virus.
Virus Research. 170, 138–149


Murtaugh, M.L., Fanning, S.W., Sharma, T.M., Terry, A.M., and Horn. J.R (2011)
A Combinatorial Histidine Scanning Library Approach to Engineer Highly pH-Dependent Protein Switches
Protein Science. 20 (9), 1619-1631 (Featured Cover Art)


Fanning, S.W. and Horn, J.R. (2011)
An Anti-Hapten Camelid Antibody Reveals a Cryptic Binding Site with Significant Energetic Contributions from a Non-Hypervariable Loop.
Protein Science. 20 (7), 1196–1207. (Featured Cover Art)

Fanning, S.W., Murtaugh, M., and Horn, J.R. (2011)
A Combinatorial Approach to Engineer a Dual-Specific Metal Switch Antibody”
Biochemistry, 50 (23), pp 5093–5095.

Franco, E.J., Sonneson,G.J., DeLegge, T.J., Hofstetter,H, Horn, J.R. and Hofstetter, O. (2010)
Production and characterization of a genetically engineered anti-caffeine camelid antibody and its use in immunoaffinity chromatography
J Chromatography B, 878, 177-186

Sonneson, G.J. and Horn, J.R. (2009)
Hapten Induced Dimerization of a Single-Domain VHH Camelid Antibody
Biochemistry. 48, 6693–6695

Horn, J.R., Sosnick, T.R., and Kossiakoff, A.K. (2009)
Principal determinants leading to transition state formation of a protein-protein complex, orientation trumps side chain interactions.
PNAS. 106, 2559-2564

Rich, R.L., et. al. (2009)
A global benchmark study using affinity-based biosensors
Analytical Biochemistry. 386, 194-216

Horn, J.R., Kraybill, B., Petro, E., Coales, S.J., Hamuro, Y., and Kossiakoff, A.K. (2006)
The role of protein dynamics in increasing binding affinity for an engineered protein-protein interaction established by H/D exchange mass spectrometry
Biochemistry. 45: 8488-8498

Kouadio, J.-L., Horn, J.R., Pal, G., and Kossiakoff, A.K. (2005)
Shotgun Alanine Scanning Shows That Growth Hormone Can Bind Productively to Its Receptor through a Drastically Minimized Interface.
J. Biol. Chem. 280: 25524-25532


Horn, J.R. and Shoichet, B.K. (2004)
Allosteric Inhibition through Core Disruption.
J.Mol.Biol. 336: 1283-91

Horn, J.R., Ramaswamy, S., Murphy, K.P. (2003)
Structure and Energetics of Protein-Protein Interactions: The Role of Conformational Heterogeneity in OMTKY3 Binding to Serine Proteases.\\ J.Mol.Biol. 331: 497-508

Horn, J.R., Brandts, J, and Murphy, K.P. (2002)
Van’t Hoff and Calorimetric Enthalpies II: Effects of Linked Equilibria.
Biochemistry 41:7501-7507

Horn, J.R., Russel, D.J., Lewis, E.A., and Murphy, K.P. (2001)
Van’t Hoff and calorimetric enthalpies from isothermal titration calorimetry: are there significant discrepancies?
Biochemistry 40:1774-8

Murphy, K.P., Baker, B.M., Edgcomb, S.P, and Horn, J.R. (1999)
Structural Energetics of Serine Protease Inhibition.
Pure Appl. Chem. 71, 1207-1213